Catalog Number - 68522-05P-2

68522-05P-2 Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering consistent performance and enhanced selectivity for challenging separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.

The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it particularly effective for halogenated, aromatic, and polar analytes. It is also suitable as an alternate column for USP methods requiring L43 classification.

With a 5 µm particle size, this column delivers lower backpressure compared to smaller particle columns, allowing higher flow rates and improved robustness while maintaining excellent chromatographic performance.

50 mm Length – Fast Screening and Rapid LC‑MS Method

The 50 mm length is optimized for speed and efficiency, making it ideal for high‑throughput environments and rapid screening applications.

• Fast analysis times for increased sample throughput

• Reduced solvent consumption

• Ideal for method scouting and rapid separations

✅ Recommended for LC‑MS workflows and fast analytical screening

Flow Rate & System Requirements (2.1 mm ID, 5 µm)

⚠️ Designed for low‑flow LC‑MS applications with improved pressure tolerance

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Lower backpressure than 3 µm columns

• Compatible with LC‑MS and low‑flow HPLC systems

• Improved robustness for routine operation

✅ Important: Standard HPLC systems must be capable of stable, accurate low flow rates

✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection

Why Choose PFP (F5) Columns for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with fluorinated and halogenated analytes

• Improved selectivity vs C18 columns

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility for method development

100 Å Pore Structure

• Suitable for small to moderately sized molecules

• Broader application range vs 60 Å columns

Cogent RP PFP™ Column Advantages

Fully End‑Capped Surface

• Minimizes silanol activity

• Improves peak symmetry

Robust Performance (5 µm Particles)

• Lower backpressure compared to smaller particles

• Reliable and consistent results

High Purity Silica

• Reproducible chromatographic performance

• Long column lifetime

Typical Applications

• Halogenated compound screening

• Aromatic compound separations

• Rapid LC‑MS workflows

• Method development and optimization

• USP L43 methods

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Designed for LC‑MS and low‑flow HPLC systems (2.1 mm ID)

• Lower backpressure compared to smaller particle sizes

• Requires accurate low‑flow pump performance

• Use 0.2 µm filtration prior to injection